ROTOR UND VERFAHREN ZUR AUTOMATISCHEN VERARBEITUNG VON FLÜSSIGKEITEN FÜR LABOR- UND BIOANALYTISCHE ZWECKE
A method is provided for carrying out an assay using a rotor assembly (220) comprising a rotor body (222) which is rotatable about an axis of rotation. The rotor body (222) has a central chamber (234) and six chambers (236 to 246) which are in communication with and radiate from the central chamber...
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Zusammenfassung: | A method is provided for carrying out an assay using a rotor assembly (220) comprising a rotor body (222) which is rotatable about an axis of rotation. The rotor body (222) has a central chamber (234) and six chambers (236 to 246) which are in communication with and radiate from the central chamber (234), and the rotor assembly (220) includes a shuttle (224) which is movable through said central chamber (234) into operative registry with any of said six chambers (236 to 246). The shuttle (224) comprises a reaction cup (252) carrying an immobilized antigen or an immobilized antibody for transport among said chambers. The method involves introducing an amount of whole blood into the first chamber (238), wash solution into the second chamber (240), an enzyme conjugate into the fourth chamber (240), an enzyme substrate solution into the fifth chamber and a reagent reference into the sixth chamber. The third chamber (236) is used for drying. The shuttle is moved between the chambers in a specified manner, the rotor body being rotated at various stages in the process, and a product is generated in the fifth chamber for comparison with the reagent reference in the sixth chamber. |
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