VERFAHREN ZUR FERMENTATIVEN HERSTELLUNG UND ISOLIERUNG VON CYCLOSPORIN A SOWIE NEUE CYCLOSPORIN- BILDENDE STÄMME

The invention relates to a method for the production by fermentation, and isolation of cyclosporin A by aerobic fermentation of a strain of the fungal species Sesquicilliopsis rosariensis G.ARNOLD or of a variant strain obtained therefrom by mutagenesis and/or selection. The biosynthesis takes place...

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Bibliographische Detailangaben
Hauptverfasser:	BORMANN, ERNST-JOACHIM DR, RUDAT, WOLF-RUEDIGER DR, ARNOLD, GUENTER DR
Format:	Patent
Sprache:	ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a method for the production by fermentation, and isolation of cyclosporin A by aerobic fermentation of a strain of the fungal species Sesquicilliopsis rosariensis G.ARNOLD or of a variant strain obtained therefrom by mutagenesis and/or selection. The biosynthesis takes place in a medium which contains ammonium salts as sole source of nitrogen, plus citrate besides other sources of carbon. A filtration aid is added to the culture suspension, which is then filtered, and the mixture of moist biomass and filtration aid thus obtained is dried, extracted with a lower carboxylic ester or a supercritical gas, preferably CO2, and the extract is degreased and chromatographed on silica gel and/or alumina or by preparative HPLC.