TRENNUNG VON ANTIKÖRPERN GEGEN METALLCHELATE

The present invention provides a method for the separation of anti-metal chelate antibodies from non-specific proteins, including antibodies, by applying a preparation containing the anti-metal chelate antibodies to an oxo acid derivatized solid support and eluting first with an elution buffer conta...

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Bibliographische Detailangaben
Hauptverfasser: JUE, RODNEY ALAN, BEIDLER, DANIEL E
Format: Patent
Sprache:ger
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Beschreibung
Zusammenfassung:The present invention provides a method for the separation of anti-metal chelate antibodies from non-specific proteins, including antibodies, by applying a preparation containing the anti-metal chelate antibodies to an oxo acid derivatized solid support and eluting first with an elution buffer containing sufficient salt concentration to elute non-specific proteins but not sufficient to elute the anti-metal chelate antibodies and then eluting the anti-metal chelate antibodies.