VERFAHREN ZUR HERSTELLUNG TIERÄRZTLICHER ACELLULARER IMPFSTOFFE GEGEN GRAMNEGATIVE MOMENTERISCHE PATHOGENE BAZILLEN

VERFAHREN ZUR HERSTELLUNG TIERÄRZTLICHER ACELLULARER IMPFSTOFFE GEGEN GRAMNEGATIVE MOMENTERISCHE PATHOGENE BAZILLEN

Surface polysaccharide and somatic polysaccharide are recovered from gram-negative nonenteric pathogenic bacilli in purified forms that are useful as veterinary acellular vaccines either individually or combined. The surface polysaccharide recovery process involves shearing the surface polysaccharid...

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Bibliographische Detailangaben
Hauptverfasser: VEDROS, NEYLAN, A, LIU, TE-NING, ERNIE
Format: Patent
Sprache:ger
Schlagworte:
CHEMISTRY
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
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Zusammenfassung:Surface polysaccharide and somatic polysaccharide are recovered from gram-negative nonenteric pathogenic bacilli in purified forms that are useful as veterinary acellular vaccines either individually or combined. The surface polysaccharide recovery process involves shearing the surface polysaccharide and somatic polysaccharide from the cell membrane, removing the bacteria, isolating the surface polysaccharide by precipitation with Cetavlon, solubilizing the precipitate, and removing nucleic acids by ethanol precipitation. The somatic polysaccharide is recovered from the supernatant remaining after the Cetavlon precipitation by ethanol precipitation.