VERFAHREN ZUR FREISETZUNG VON POLY-3-HYDROXYCARBONSAEUREN

Procedure for releasing poly-3-hydroxy carboxylic acid granules from cells of Gram-negative bacteria containing these by expression of a cloned natural or chimeric lysis gene, in which before induction of lysis gene expression the ionic strength of doubly charged cations is increased to 0.05 to 0.5...

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1. Verfasser:	LUBITZ WERNER DR
Format:	Patent
Sprache:	ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Procedure for releasing poly-3-hydroxy carboxylic acid granules from cells of Gram-negative bacteria containing these by expression of a cloned natural or chimeric lysis gene, in which before induction of lysis gene expression the ionic strength of doubly charged cations is increased to 0.05 to 0.5 mol, expression of the lysis gene is induced by increasing the temperature, the cells are harvested and the still moist cells are resuspended in water or buffer solutions, whereupon the polymer granules are released by spontaneous lysis.