Esterase from Pseudomas marginata

The present invention relates to a novel esterase which has been made available by genetic engineering strategies. The esterase described in this invention is active on a number of carboxylic esters including triglycerides, fatty acid nitrophenyl esters and various esters with secondary alcohols. It...

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Bibliographische Detailangaben
Hauptverfasser:	GRIENGL HERFRIED. DR, KLEMPIER NORBERT DR, SCHWAB HELMUT. DR, FABER KURT. DR, STUBENRAUCH GERHARD. DR
Format:	Patent
Sprache:	eng ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a novel esterase which has been made available by genetic engineering strategies. The esterase described in this invention is active on a number of carboxylic esters including triglycerides, fatty acid nitrophenyl esters and various esters with secondary alcohols. It is possible by cloning the corresponding gene from the bacterium Pseudomonas marginata, strain ATCC 10248, and overexpression of the gene in microorganisms to produce active enzyme preparations. Such enzyme preparations can preferably be used in organic synthesis for ester cleavages and transesterifications, it being possible where appropriate to achieve enantioselectivity in the conversions.