Extraction and quantification of DNA from touched cell phones surfaces
Touched mobile surfaces became part of crime scene evidences which analyzed by forensic scientists. The minimal amount of DNA required for full STR profile is 0.2-0.5ng. The aim of this work is to quantitate the amount of DNA isolated from three touched mobiles brands (iPhone, Samsung, and Sony) sur...
Gespeichert in:
| Veröffentlicht in: | Iraqi Journal of Biotechnology. 2019, Vol.18 (3), p.85-89 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 89 |
|---|---|
| container_issue | 3 |
| container_start_page | 85 |
| container_title | Iraqi Journal of Biotechnology. |
| container_volume | 18 |
| creator | Sabah, Majid Arshid Rashid, Sahir Nimah, Duha Salim Shihab, Maryam Jasim Salih, Zual-nun Yunus Naneel, Sura Husam, Rim |
| description | Touched mobile surfaces became part of crime scene evidences which analyzed by forensic
scientists. The minimal amount of DNA required for full STR profile is 0.2-0.5ng. The aim of this work is
to quantitate the amount of DNA isolated from three touched mobiles brands (iPhone, Samsung, and
Sony) surfaces using Organic and Chelex DNA extraction methods. The results showed that organic
method extract more DNA than Chelex from Samsung mobile surface and fewer amounts from iPhone
and Sony. Quantification of DNA by Real time PCR was more accurate with quantities near the minimal
amount required for full profile analysis. STR analysis showed a partial DNA profile recovered from all
cell phones brands. The results indicate the possibility of obtain suitable quantities of DNA from cell
phone surface |
| format | Article |
| fullrecord | <record><control><sourceid>emarefa</sourceid><recordid>TN_cdi_emarefa_primary_955874</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>955874</sourcerecordid><originalsourceid>FETCH-emarefa_primary_9558743</originalsourceid><addsrcrecordid>eNqFiUELgjAYQEcUJOVPCL4_IMy1oR6jlE6dusvn3HCgm20T6t8X0b3Te7y3IgkTjGYF42JNkrzMRcaLim9JGoLpaF5WTFAqEtLUz-hRRuMsoO3hsaCNRhuJ3-Q0XG4n0N5NEN0iB9WDVOMI8-CsChAWr1GqsCcbjWNQ6Y87cmjq-_maqQm90tjO3nzs1VZClAU__vtvc605iw</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Extraction and quantification of DNA from touched cell phones surfaces</title><source>EZB-FREE-00999 freely available EZB journals</source><creator>Sabah, Majid Arshid ; Rashid, Sahir ; Nimah, Duha Salim ; Shihab, Maryam Jasim ; Salih, Zual-nun Yunus ; Naneel, Sura ; Husam, Rim</creator><creatorcontrib>Sabah, Majid Arshid ; Rashid, Sahir ; Nimah, Duha Salim ; Shihab, Maryam Jasim ; Salih, Zual-nun Yunus ; Naneel, Sura ; Husam, Rim</creatorcontrib><description>Touched mobile surfaces became part of crime scene evidences which analyzed by forensic
scientists. The minimal amount of DNA required for full STR profile is 0.2-0.5ng. The aim of this work is
to quantitate the amount of DNA isolated from three touched mobiles brands (iPhone, Samsung, and
Sony) surfaces using Organic and Chelex DNA extraction methods. The results showed that organic
method extract more DNA than Chelex from Samsung mobile surface and fewer amounts from iPhone
and Sony. Quantification of DNA by Real time PCR was more accurate with quantities near the minimal
amount required for full profile analysis. STR analysis showed a partial DNA profile recovered from all
cell phones brands. The results indicate the possibility of obtain suitable quantities of DNA from cell
phone surface</description><identifier>ISSN: 1815-4794</identifier><identifier>EISSN: 2520-7245</identifier><language>eng</language><publisher>Baghdad, Iraq: University of Baghdad, Genetic Engineering and Biotechnology for Postgraduate Studies</publisher><ispartof>Iraqi Journal of Biotechnology., 2019, Vol.18 (3), p.85-89</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Sabah, Majid Arshid</creatorcontrib><creatorcontrib>Rashid, Sahir</creatorcontrib><creatorcontrib>Nimah, Duha Salim</creatorcontrib><creatorcontrib>Shihab, Maryam Jasim</creatorcontrib><creatorcontrib>Salih, Zual-nun Yunus</creatorcontrib><creatorcontrib>Naneel, Sura</creatorcontrib><creatorcontrib>Husam, Rim</creatorcontrib><title>Extraction and quantification of DNA from touched cell phones surfaces</title><title>Iraqi Journal of Biotechnology.</title><description>Touched mobile surfaces became part of crime scene evidences which analyzed by forensic
scientists. The minimal amount of DNA required for full STR profile is 0.2-0.5ng. The aim of this work is
to quantitate the amount of DNA isolated from three touched mobiles brands (iPhone, Samsung, and
Sony) surfaces using Organic and Chelex DNA extraction methods. The results showed that organic
method extract more DNA than Chelex from Samsung mobile surface and fewer amounts from iPhone
and Sony. Quantification of DNA by Real time PCR was more accurate with quantities near the minimal
amount required for full profile analysis. STR analysis showed a partial DNA profile recovered from all
cell phones brands. The results indicate the possibility of obtain suitable quantities of DNA from cell
phone surface</description><issn>1815-4794</issn><issn>2520-7245</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNqFiUELgjAYQEcUJOVPCL4_IMy1oR6jlE6dusvn3HCgm20T6t8X0b3Te7y3IgkTjGYF42JNkrzMRcaLim9JGoLpaF5WTFAqEtLUz-hRRuMsoO3hsaCNRhuJ3-Q0XG4n0N5NEN0iB9WDVOMI8-CsChAWr1GqsCcbjWNQ6Y87cmjq-_maqQm90tjO3nzs1VZClAU__vtvc605iw</recordid><startdate>2019</startdate><enddate>2019</enddate><creator>Sabah, Majid Arshid</creator><creator>Rashid, Sahir</creator><creator>Nimah, Duha Salim</creator><creator>Shihab, Maryam Jasim</creator><creator>Salih, Zual-nun Yunus</creator><creator>Naneel, Sura</creator><creator>Husam, Rim</creator><general>University of Baghdad, Genetic Engineering and Biotechnology for Postgraduate Studies</general><scope>ADJCN</scope><scope>AHFXO</scope></search><sort><creationdate>2019</creationdate><title>Extraction and quantification of DNA from touched cell phones surfaces</title><author>Sabah, Majid Arshid ; Rashid, Sahir ; Nimah, Duha Salim ; Shihab, Maryam Jasim ; Salih, Zual-nun Yunus ; Naneel, Sura ; Husam, Rim</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-emarefa_primary_9558743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><toplevel>online_resources</toplevel><creatorcontrib>Sabah, Majid Arshid</creatorcontrib><creatorcontrib>Rashid, Sahir</creatorcontrib><creatorcontrib>Nimah, Duha Salim</creatorcontrib><creatorcontrib>Shihab, Maryam Jasim</creatorcontrib><creatorcontrib>Salih, Zual-nun Yunus</creatorcontrib><creatorcontrib>Naneel, Sura</creatorcontrib><creatorcontrib>Husam, Rim</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><jtitle>Iraqi Journal of Biotechnology.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sabah, Majid Arshid</au><au>Rashid, Sahir</au><au>Nimah, Duha Salim</au><au>Shihab, Maryam Jasim</au><au>Salih, Zual-nun Yunus</au><au>Naneel, Sura</au><au>Husam, Rim</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extraction and quantification of DNA from touched cell phones surfaces</atitle><jtitle>Iraqi Journal of Biotechnology.</jtitle><date>2019</date><risdate>2019</risdate><volume>18</volume><issue>3</issue><spage>85</spage><epage>89</epage><pages>85-89</pages><issn>1815-4794</issn><eissn>2520-7245</eissn><abstract>Touched mobile surfaces became part of crime scene evidences which analyzed by forensic
scientists. The minimal amount of DNA required for full STR profile is 0.2-0.5ng. The aim of this work is
to quantitate the amount of DNA isolated from three touched mobiles brands (iPhone, Samsung, and
Sony) surfaces using Organic and Chelex DNA extraction methods. The results showed that organic
method extract more DNA than Chelex from Samsung mobile surface and fewer amounts from iPhone
and Sony. Quantification of DNA by Real time PCR was more accurate with quantities near the minimal
amount required for full profile analysis. STR analysis showed a partial DNA profile recovered from all
cell phones brands. The results indicate the possibility of obtain suitable quantities of DNA from cell
phone surface</abstract><cop>Baghdad, Iraq</cop><pub>University of Baghdad, Genetic Engineering and Biotechnology for Postgraduate Studies</pub><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1815-4794 |
| ispartof | Iraqi Journal of Biotechnology., 2019, Vol.18 (3), p.85-89 |
| issn | 1815-4794 2520-7245 |
| language | eng |
| recordid | cdi_emarefa_primary_955874 |
| source | EZB-FREE-00999 freely available EZB journals |
| title | Extraction and quantification of DNA from touched cell phones surfaces |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T07%3A26%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-emarefa&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Extraction%20and%20quantification%20of%20DNA%20from%20touched%20cell%20phones%20surfaces&rft.jtitle=Iraqi%20Journal%20of%20Biotechnology.&rft.au=Sabah,%20Majid%20Arshid&rft.date=2019&rft.volume=18&rft.issue=3&rft.spage=85&rft.epage=89&rft.pages=85-89&rft.issn=1815-4794&rft.eissn=2520-7245&rft_id=info:doi/&rft_dat=%3Cemarefa%3E955874%3C/emarefa%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |