Extraction and quantification of DNA from touched cell phones surfaces
Touched mobile surfaces became part of crime scene evidences which analyzed by forensic scientists. The minimal amount of DNA required for full STR profile is 0.2-0.5ng. The aim of this work is to quantitate the amount of DNA isolated from three touched mobiles brands (iPhone, Samsung, and Sony) sur...
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Veröffentlicht in: | Iraqi Journal of Biotechnology. 2019, Vol.18 (3), p.85-89 |
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Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Touched mobile surfaces became part of crime scene evidences which analyzed by forensic
scientists. The minimal amount of DNA required for full STR profile is 0.2-0.5ng. The aim of this work is
to quantitate the amount of DNA isolated from three touched mobiles brands (iPhone, Samsung, and
Sony) surfaces using Organic and Chelex DNA extraction methods. The results showed that organic
method extract more DNA than Chelex from Samsung mobile surface and fewer amounts from iPhone
and Sony. Quantification of DNA by Real time PCR was more accurate with quantities near the minimal
amount required for full profile analysis. STR analysis showed a partial DNA profile recovered from all
cell phones brands. The results indicate the possibility of obtain suitable quantities of DNA from cell
phone surface |
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ISSN: | 1815-4794 2520-7245 |