Isolation of jasmimin from jasmine (Jasminum sambac)

Jasminum sambac (oleaceae) cultivated in Iraq, it is an important medicinal plant, that is used as antimicrobial, anticancer and antioxidant. In previous study, Jasminin compound was extracted, isolated and identificated from chloroform, 100% methanol, 70% methanol and petroleum ether which extracted from whole dry plant (root, stem, leaf and flower). Quantitative and qualitative test, such as special reagent, TLC and HPLC tests were done. The result showed that chloroform and 70% methanol extract had the ability to extracted jasminin compound from plant parts than any other organic solvent. The qualitative data revealed that the retreadactin factor Rf value of jasminin was (0.8), quantitative test (HPLC) show that methanolic and chloroformic extract had (0.62696 mg) and (1.11386 mg) jasminin for one gram dry plant respectively. Jasminin purified from chloroformic and methanolic extract using preparative TLC (0.75 cm), the HPLC for the purified jasminin showed one peak respectively