عزل بكتريا Clostridium botulinum من مصادر متنوعة و تشخيصها

Sixteen local isolates of Clostridium were isolated from sources: Soil, meat and honey. Those sources were obtained from different districts in Basrah government. Characterization of isolates were made after purification by using morphological and biochemical tests which revealed that the isolates were Clostridium botulinum. The microscopic test appeared that all isolates are bacilli, gram positive, obligate anaerobic ,forming spores and motile. There's colonies had wide spread form with uniform border when grown of blood agar and egg yolk agar and looked transparent to gray. The biochemical tests revealed that the isolates are β-heamolysis, lipid hydrolyzed, produced H2S, all of them were negative in indole production, nitrate reduction, starch hydrolysis and catalase production. They were grown in pH (4-6), temperature (10, 30,45)°C and NaCl (3-12)%. They fermented all the sugars to jog lactose, galactose, amygdalin, Eunosetol and ribose. They were sensitive for some antibiotics like Erethromycin, Refampin, Metronidazol and Penicillin, Penicillin but they were resistant for, Gentamicin, Nalidixic acid and Trimethoprim.