Expression of toll-like receptor 2 and 4 from patients with chronic obstructive pulmonary disease in peripheral blood neutrophil cells
Objectives: Chronic obstructive pulmonary disease (COPD) is one of the leading causes of morbidity and mortality around the world. Preliminary studies have evaluated the association between innate immunity including Toll-like receptors (TLRs) and airway samples of patients with COPD. The role of TLR...
Gespeichert in:
Veröffentlicht in: | Oman medical journal 2017, Vol.32 (6), p.417-425 |
---|---|
Hauptverfasser: | , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Objectives: Chronic obstructive pulmonary disease (COPD) is one of the leading causes
of morbidity and mortality around the world. Preliminary studies have evaluated the
association between innate immunity including Toll-like receptors (TLRs) and airway
samples of patients with COPD. The role of TLRs in peripheral blood neutrophils is
poorly understood. Hence, this study aimed to investigate the role of TLR2 and TLR4
in peripheral blood neutrophils of COPD patients. Methods: A total of 101 COPD
cases and an equal number of healthy controls participated in this case-control study.
Peripheral blood neutrophils were isolated from all participants and cultured for 24 hours
through lipopolysaccharide (LPS) stimulation. The gene expressions of TLR2 and TLR4
were assessed by real-time polymerase chain reaction. The protein levels of interleukin
(IL)-8 and matrix metalloproteinase (MMP)-9 were measured in neutrophils cell culture
supernatants using enzyme-linked immunosorbent assay (ELISA). Results: The levels
of IL-8 and MMP-9 were significantly higher in patients with COPD compared to
healthy controls. Similarly, the gene expression of TLR2 and TLR4 were increased
in LPS stimulated peripheral blood neutrophils of patients with COPD. Smoke pack
years was positively correlated with IL-8 levels and negatively correlated with forced
expiratory volume in the first second % (r = -0.33; p = 0.023) and FEV1/forced vital
capacity (FVC) (r = -0.27; p = 0.011). Conclusions: The increased expression of TLR2
and TLR4 suggests its role in disease pathogenesis of COPD. Smoke pack years was
negatively associated with spirometric parameters in COPD patients. This may help to
predict the smokers without COPD who risk developing the condition in the future |
---|---|
ISSN: | 1999-768X 2070-5204 |