The role of harmane and norharmane in in vitro dopaminergic function
Background. Nicotine is one of the most frequently used drugs of abuse. It has long been speculated that in addition to nicotine, tobacco contains other compounds that reinforce addiction. Two promising candidates are the tobacco smoke constituents, harmane and norharmane. Purpose. Our study evaluat...
Gespeichert in:
Veröffentlicht in: | Journal of drug and alcohol research 2015, Vol.4 (2015), p.1-8 |
---|---|
Hauptverfasser: | , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Background. Nicotine is one of the most frequently used
drugs of abuse. It has long been speculated that in addition to nicotine,
tobacco contains other compounds that reinforce addiction. Two
promising candidates are the tobacco smoke constituents, harmane and
norharmane. Purpose. Our study evaluated the effects of exposure to
harmane and norharmane alone and in combination with nicotine on
midbrain neuronal cultures. Experimental design. After 24 h exposure,
cytotoxicity, dopamine (DA) levels, pTH, TH, MAO-A, and MAO-B
expression levels were investigated. Results. At low-to-moderate doses
(1 μM–100 μM), harmane or norharmane exposure caused significant
dose-dependent decreases in intracellular DA without causing high rate
of cell death, mitochondrial dysfunction or generation of ROS. Harmane
exposure also resulted in subsequent increase in extracellular DA
levels. Conclusion. Along with downregulation of protein expression,
notably MAO-B, our data indicate the potential of harmane and norharmane
to act in synergy with nicotine in midbrain neurons |
---|---|
ISSN: | 2090-8334 2090-8342 |