Effect of goat breeds, semen diluents and freezing methods on sperm freezability and reproductive performance

Two experiments were designed to study the effect of semen extenders and freezing regimens on post-thaw semen motility and viability index, effect of bucks breed was also included (experiment 1). In experiment 2, semen diluents effects on reproductive performance was conducted. Semen was collected from bucks breeds (Aradi (A), Damascuss (D) and cross (½A½D). Good quality semen was divided into 4 portions, each diluted with one diluent (Milk, Na.Citrate, Tris and Na.Bicarbonate). The diluted semen was packaged into 0.5ml straws then cooled to 5°C. After equilibration, half of the packaged straws were suspended 15cm above liquid nitrogen (LN) for 15min (Freezing regimen1; slow). Other half of straws was suspended at height 15 and 5cm of LN for 10 and 5min, respectively (Freezing regimen2; rapid) before plunged into LN. Frozen semen was thawed for post-thaw motility and viability. In the second experiment, semen with good quality was extended with three types of extenders (Milk, Na.Citrate and Tris). Diluted semen were cooled to 5°C and used for AI. Results revealed that, pre-freeze semen motility was significant higher in Tris, Na.Citrate and Na.Bicarbonate than milk diluent. Post-thaw semen motility and viability were highly significant for milk and Na.Citrate than Tris and Na.Bicarbonate diluents. Post-thaw semen motility was significantly higher in Aradi and Damascus than cross breed. Post-thaw semen motility and viability revealed, significant higher means for Freezing regimen 2 than regimen 1. Milk was significantly higher than Tris and Na.Citrate diluents for fertility and fecundity. It was concluded that, regarding to post-thaw semen viability, fertility%, kidding%, fecundity% and prolificacy%, milk is preferable than Tris and Na.citrate diluents. الهدف من هذه الدراسة هو معرفة تأثير ممددات السائل المنوي و طرق تجميده و سلالة التيوس على حركة و حيوية الحيامن بعد التجميد و الإسالة و كذلك تأثير نوع المخف المستخدم في التلقيح الاصطناعي على الكفاءة التناسلية للماعز. و لتحقيق هذه الأهداف صممت تجربتين : في التجربة الأولى : تم تجميع السائل المنوي من تيوس لثلاثة سلالات من الماعز هي العارضى و الدمشقي و الخليط، قسمت العينات على أربعة أجزاء، كل جزء تم تخفيفه بنوع من الممدات هي الحليب منزوع الدسم و الترس و سترات الصوديوم و أخيراً مخفف بيكربونات الصوديوم. بعد التخفيف تم تعبئة السائل المنوي في قصيبات سعة 2/1 مللى ثم بردت تدريجياً و تركت في الثلاجة لمدة لم تقل عن ساعتين لإعطاء فرصة للحيامين للتأقلم و التكييف مع المخفف في درجة 5 مئوية، بعد هذه الفترة تم تقسيم القصيبات إل