Detection of plasmid profile coding antibiotic resistance of klebsiella spp. isolated from different infection

One hundred and fifty five of Gram-negative-lactose fermented bacteria grown on MacConkey agar were collected from burns, wounds, UTI and RTI in two hospitals of Najaf province. The isolates were identified according to cultural characteristics and biochemical activities. 43 (27.7 %) isolates were Klebsiella spp. represented by 35 (81.3%) were K. pneumonia subsp. aerogenes, 4 (9.3 %) was Klebsiella pneumoniae subsp. pneumoniae, 2 (4.6 %) was Klebsiella oxytoca, as well as one isolate for each of K. pneumoniae subsp. ozaenae and K. pneumoniae subsp. Rhinoscleromatis. Plasmid profiles of all Klebsielae isolates were studied and the results have revealed that 37 (86 %) isolates had two distinct plasmid bands.