الفعالية التثبيطية لبيتاكلوكان الشعير و استخدامه محفزا حيوياً في الحليب الاسيدوفيلي

β-glucan was extracted from local barley bran (Hordeum vulgar L) cv.Eba 265 , by water extraction method at pH 7and 55 C˚.Its molecular weight was estimated by size exclution HPLC,which reached 92×103 kd compared to standard . Inhibition effects of β-glucan toward different microorganisms was examined to assess the antimicrobial activity . The microorganism included Staphylococcus aureus , Bacillus cereus , Escherichia coli , Pseudmonas aeruginosa . Inhibition zone diameters at 0.1 mg/ml were 19,15,10and 9 mm ,respectively ,at 0.2 mg/ml concentration β-glucan were 21,17.5, 12 and 11 mm respectively , β-glucan extract at 0.1 ,0.2 concentration have no inhibition activity towards Rhizopus stolonifer and Saccharomyces cerevisiae. The effect of β-glucan in Lactobacillus acidophilus growth was studied to illustrate its role as prebiotic. Adding 1,2,3 % β-gluan to acidophilus milk product produced by Lactobacillus acidophilus starter showed significant increase in bacteria number during storage for 24 hours which were 10.70,10.66 ,10.60 respectively compared to control treatment 10.50 and there were a decrease in pH value for all treatments which reached 4.7, 4.6 ,4.4 respectively compared to control treatment 5 of acidophilus milk .Hence consumed the whole grain the highest contained percentage of β- gluan such as Barley and Oat and used in prepare kinds of food and study important in stimulation of immunity against diseases by using experimental study in vitro and in vivo also other study on antimicrobial activity against other bacteria and important in enhance the immune response for human against diseases . β-gluan can use in different concentration such as prebiotic for LBA and range effect of added different concentration from β-gluan on another kind of LBA also other experimental study around consumed β-gluan from microflora . *Part of MS.c.Thesis of first author استخلص البيتاكلوكان من نخالة حبوب الشعير صنف اباء 265 باستخدام طريقة الاستخلاص المائي في رقم هيدروجيني 7 باستخدام كربونات الصوديوم و بدرجة حرارة 55م. قدر وزنه الجزئي باستخدام تقانة HOLC. حيث بلغ الوزن الجزيئي للبيتاكلوكان 103x 92 كليودالتون. اختبرت الفعالية التثبيطية للبيتاكلوكان الخام المستخلص وبتركيزي 0.1 و 0.2 ملغم / مل اتجاه 4 أنواع من البكتيريا هي Pseudomonas aeruginosa, Escherichia coli, Bacillus cereus, Staphylococcus aureus بطريقة الانتشار في الحفر Cup assay. أظهرت النتائج أن قطر هالة التثبيط عند تركيز 0.1 ملغم / مل هي 19 و 15 و 10 و 9 ملم على الترتيب أما عند تركيز 0.2 ملغم / مل 21 و 17.5