تأثير إضافة مسحوق الثوم Allium sativum إلى عليقة فروج اللحم المخمجة تجريبيا بالفايروس المسبب لمرض النيوكاسل

This study included the effect of garlic powder (Allium sativum) on gross and Histopathological changes in brain, trachea, proventiculus, intestine and cecal tonsil induced by Newcastle disease virus (NDV). The study also contributed the effect of garlic powder as an Immunomodulator through demonstrating the stress index and serum antibody levels against NDV estimated by HI, the study also detected the antioxidant effect of garlic powder by estimation of Peroxynitrite in serum in english Ross broiler chickens. Chicks at one day old have been divided into 5 groups randomly with 50 chicks for each. First group fed on diet containing 1% garlic powder from first day (group A), the second fed on 1% garlic powder diet from first day and infected with 0.1 ml ND virus at concentration 1x106 EID50 orally at 14 days old (group B), the third fed on garlic powder and infected with ND virus at 14 days old (group C), the fourth did not fed on garlic powder but infected with ND virus at 14 days old (group D) where used as untreated negative control. The results revealed occurrence of clinical, pathological changes with mortality after 72hr post infection at group D which represented by respiratory signs as gasping, nasal discharge with nervous signs include torticulus, wings and legs paralysis, less sever form occur in group C and mild form in group B. The Gross lesions included congestion, hemorrhage in brain, congestion of trachea, petechial hemorrhage in proventiculus, hemorrhage and ulceration of cecal tonsil in group D and these lesions are less sever in group C and mild in group B, but have not been observed in group A. Histopathological changes included vacculation and demylation with perivascular cuffing of lymphocyte, congestion blood vessels in brain, in trachea there was hemorrhage and degeneration of epithelial cells, in proventiculus there was infiltration of inflammatory cells in gastric glands and muscular layer with congestion of blood vessels at group D, but less severly observed at group C and less frequently and less severity at group B, it is not observed at group A and control, this changes was not observed at group A and control. The results indicated a significant decrease of stress index and serum antibody levels at group B and C compared with group D, The result also showed a significant decrease of peroxynitrate radical in group A compare with other groups. We concluded that administration of garlic powder with diet of chicks from first day redu