تأثير عزلات البكتيريا Pseudomonas fluorescens والبكيتريا المحلية Bacillus thuringiensis tenebrionis وجسيمات الفضة النانوية المحضرة حيوياً في فاعلية أنزيم السيليلوليز في الجهاز الهضمي الشغالات الأرضة Microcerotermes diversus Silly

The purpose of this study was to investigate the effect of different bacterial isolates of P. fluorescens and B. 1. tenebrionis and silver nanoparticles on the activity of the cellulase enzyme in termites' middle digestive system and its role in glucose release by treating cellulose media with bacterial strains and nanoparticles. Ultraviolet spectroscopy results revealed that silver nanoparticles synthesized by P. fluorescens isolated from termites, commercial P. fluorescens, and local B.t. tenrbrionis had the highest absorption at 385, 324, and 324 nm wave length, respectively. Infrared spectroscopy (FTIR) revealed that ketons, amines, alkanes, and alcohols were among the groups that contributed to silver ions reduction, capping and stabilizing nanoparticles. The particle diameters were measured using a scanning electron microscope (SEM) and found to be on average 32.43, 63.19, and 57.62 nm. The highest concentration of liberated glucose was recorded after 24 hours, which ranged between 0.453 and 1.285 mg/ml when treated with silver nanoparticles prepared from a commercial isolate of P. fluorescens bacteria and when treated with a bacterial isolate P. fluorescens, respectively. Only when treated with synthesized silver nanoparticles and when treated with commercial P. fluorescens isolates did the lowest concentrations of liberated sugar reached between 0.037 and 0.730 mg/ml after 72 hours, respectively. The high quantity of liberated sugar in the control treatment was constant, reaching 1.70 and 2.33 mg /ml after 24 and 72 hours, respectively. In addition to the variation in cellulase enzyme inhibition rates in termite digestive systems due to the effect of different treatments, it was discovered that the local isolate of B. 1. tenrbrionis was better than the rest of the isolates in affecting the enzyme, causing inhibition rates of 71.42%. Furthermore, silver nanoparticles synthesized from a commercial isolate of P. fluorescens outperformed other treatments in inhibiting enzyme activity, with an inhibition rate of 78.57%. هدفت هذه الدراسة إلى اختبار تأثير عزلات محلية مختلفة من البكتيريا P. fluorescens و B to tenebrionis. وجسيمات الفضة النانوية المحضرة بالطرائق الحيوية البيولوجية في فاعلية أنزيم السيليلوليز الموجود في الجهاز الهضمي الوسطى لشغالات الأرضة، ودوره في تحرير الجلوكوز. استخدمت 3 عزلات محلية من البكتيريا Pluorescens المعزولة من حشرات الأرضة والجراد والصرصار الأمريكي، وعزلة تجارية، فضلاً عن العزلة المحلية للبكتيريا B., tenebrionis ، كما استخدمت جسي