تقصي انتشار و التنوع ضمن مجتمع الفطر Botrytis cinerea على بعض محاصيل الخضار المحمية في الساحل السوري

Survey of gray mold on some protected vegetables along the Syrian Coast was conducted during the 2015/2016 and 2016/2017 growing seasons. The study showed the spread of B. cinerea in both seasons on various vegetable crops such as tomato, squash and eggplant. The percentage of greenhouses affected was 60.48% and 54.76% during the 2016 and 2017 seasons, respectively. B. cinerea isolates collected from different crops and sites showed a variation in their pathogenicity and severity when tested on apple fruits. High pathogenicity level appeared in two isolates collected from tomato in each of Dweer Al-Khateeb and Hrisson locations, whereas very low pathogenicity level appeared in four isolates collected from squash and eggplant in Aen Alarous, Majdaloun Albahr, Saloren and Qreir locations. Variation in pathogenicity among of studied gray mold isolates was associated with genomic variation when tested by RAPD and ISSR. For RAPD, the total amplified fragments number was 71 and contained 62 polymorphic fragments with polymorphism rate of 87.65% when 12 random primers were used. Whereas the total amplified fragments number was 48 and contained 42 polymorphic fragments with polymorphism rate 87.5% when 6 primers were used with ISSR. RAPD showed higher capacity in showing genomic variation than ISSR among B. cinerea isolates, which were distributed to six classes with RAPD and three classes with ISSR. There was no effect of crop type and geographic factor and pathogenicity on genomic variation among B. cinerea isolates.