A study relationship between IL 1β (+ 3954) gene polymorphism with moderate and sever chronic periodontitis among sample of Iraqians population in Babylon province

Intoduction: Theqcytokine, is aprimary activator of earlyqchemotacticqcytokines, as well as of expressionqof adhesionq molecules that ,facilitateqmigration of leukocytes intoqtissues.,,IL-1q is also. one of the most, activeqstimulators ,ofqosteoclastqcells ,,,and lead to boneqresorption...Theqaim ofqthis study was toqdetermined.. the .prevalenceqof the ILq1 β (q+3954.)t geneqpolymorphism in a sample of iIraqi8 patientsqwith qchronicqperiodontitisy in4 Babylon .,Province and to evaluater they association of thisopolymorphism witht the severityqof disease,,. Methodsw: Seventy four systemicallyqhealthyqpatients attending theqDepartment of Periodontics of Facultyqof Dentistry, Babylon University, BabylonqProvince, Iraq.r Patients wereqclassified into twoqgroups: subjects withqchronicqperiodontitis groupq(CP) (n=40) andq healthyqcontrolqgroup (C)..(n=34). Thisqstudy desiged a cross8sectionalqstudy.5GenomicqqDNA was obtained from venousqblood(5 ml)5 and amplifiedy usings the Polymerasey Chainu Reactionq (PCR) with specific primers flankingqthe locus *+3954 of*ILq1β. PCRq.products wereqsubmitted to restriction endonuclease digestionaand analyzed by polyacrylamideqgel electrophoresis, to distinguish allelesqTqandqCqof the ILq1 β gene, allowing for the determinationqof the genotypesqand detection qof the polymorphism. Results: The homozygousqgenotype CC of the ILz-1β( +3954) was dominatedzin controlq(C )group than in Chroniczperiodontitis (CP) group. The heterozygousqgenotype CTysignificantly higher in Chronicuperiodontitis groupzthan zyin controlzgroup,(P =0.038; OR 4.125 ). The Prevalencezof thezTT genotypezywas dominated in chronic periodontitis (CP)groupz than in controlz(C )group,and statisticallyyinsignificant valueowas obtainedpwhenpcompared betweenuthese twoogroups(P =0.27). Weuobserved that,individualsqwith severechronicq periodontitisqgroup displayedqhigh frequencyqof Tsoallele(47.6 %) as compareduto individualsqwith moderateqchronic periodontitisqgroup, at the same time no significant difference was observed between them. The chroniciqperiodontitis groupqdisplayed a higherqpercentage of the Tqtallele (45%) when compared,with,controlrogroup(29.4%6),and statistical pinsignificant in alleleqdistribution wasoobserved between these twopgroups. Conclusion:- Our dataqsuggested thatqthe polymorphismqin the locusq+3954 ofwIL1 β geneqcould be related toqchronic periodontitisqin Iraqiqpopulation. Key words: Gene6 polymorphism.,99interleukin))1 β, ,7chronic&*periodo