Optimization of Parameters for a More Efficient Use ofAdipose-Derived Stem Cells in Regenerative Medicine Therapies
Adipose tissue-derived stem cells (ASCs) association to fat in autologous lipotransfer is promising for a more effective soft tissue reconstruction, and optimization of protocols to isolate ASCs from lipoaspirate fat is much needed. We demonstrated that an increase in adipocyte differentiation is de...
Gespeichert in:
Veröffentlicht in: | Stem cells international 2012, Vol.2012 (2012), p.1-7 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 7 |
---|---|
container_issue | 2012 |
container_start_page | 1 |
container_title | Stem cells international |
container_volume | 2012 |
creator | Alonso, Nivaldo Atique, Rodrigo Ishiy, Felipe Augusto André Tissiani, Luiz Alexandre Lorico Dalto Fanganiello, Roberto Aguena, Meire Passos-Bueno, Maria Rita |
description | Adipose tissue-derived stem cells (ASCs) association to fat in autologous lipotransfer is promising for a more effective soft tissue reconstruction, and optimization of protocols to isolate ASCs from lipoaspirate fat is much needed. We demonstrated that an increase in adipocyte differentiation is dependent on the number of ASCs. In a sample of 10 donors, we found a higher concentration of nucleated cells in the lower abdomen compared to flank (P=0.015). In a sample of 6 donors we did not find differences in the cell yield obtained by manual or pump-assisted aspiration (P=0.56). We suggest that the increase in the number of ASCs in the reinjected fat may enhance the efficiency of newly formed adipose tissue and that the anatomical region from which to harvest fat tissue needs to be considered to optimize the number of ASCs in the harvested tissue. Finally, pump-assisted aspiration can be used without any significant harm to the viability of cells. |
doi_str_mv | 10.1155/2012/303610 |
format | Article |
fullrecord | <record><control><sourceid>emarefa</sourceid><recordid>TN_cdi_emarefa_primary_1030007</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1030007</sourcerecordid><originalsourceid>FETCH-emarefa_primary_10300073</originalsourceid><addsrcrecordid>eNqFTsFKAzEUDKJgsT15l_cDa5ON3W2PUiteimIreCvBneiT3WTJCwX9enMQr85lhmFmGKUujb42ZrGY19rUc6ttY_SJmphm2Varpl2e_unm9VzNRD51gV3pG11PlDyOmQf-dpljoOjpySU3ICMJ-ZjI0TYm0MZ7fmOETC-CErvteIyC6g6Jj-holzHQGn0vxIGe8Y6AVDaPoC26Ug2g_UexRoZM1Zl3vWD2yxfq6n6zXz9UGFyCd4cxcVFfB6NtudrafwM_HyxNvA</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Optimization of Parameters for a More Efficient Use ofAdipose-Derived Stem Cells in Regenerative Medicine Therapies</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central Open Access</source><source>Wiley Online Library Open Access</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Alonso, Nivaldo ; Atique, Rodrigo ; Ishiy, Felipe Augusto André ; Tissiani, Luiz Alexandre Lorico ; Dalto Fanganiello, Roberto ; Aguena, Meire ; Passos-Bueno, Maria Rita</creator><creatorcontrib>Alonso, Nivaldo ; Atique, Rodrigo ; Ishiy, Felipe Augusto André ; Tissiani, Luiz Alexandre Lorico ; Dalto Fanganiello, Roberto ; Aguena, Meire ; Passos-Bueno, Maria Rita</creatorcontrib><description>Adipose tissue-derived stem cells (ASCs) association to fat in autologous lipotransfer is promising for a more effective soft tissue reconstruction, and optimization of protocols to isolate ASCs from lipoaspirate fat is much needed. We demonstrated that an increase in adipocyte differentiation is dependent on the number of ASCs. In a sample of 10 donors, we found a higher concentration of nucleated cells in the lower abdomen compared to flank (P=0.015). In a sample of 6 donors we did not find differences in the cell yield obtained by manual or pump-assisted aspiration (P=0.56). We suggest that the increase in the number of ASCs in the reinjected fat may enhance the efficiency of newly formed adipose tissue and that the anatomical region from which to harvest fat tissue needs to be considered to optimize the number of ASCs in the harvested tissue. Finally, pump-assisted aspiration can be used without any significant harm to the viability of cells.</description><identifier>ISSN: 1687-966X</identifier><identifier>EISSN: 1687-9678</identifier><identifier>DOI: 10.1155/2012/303610</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><ispartof>Stem cells international, 2012, Vol.2012 (2012), p.1-7</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids></links><search><creatorcontrib>Alonso, Nivaldo</creatorcontrib><creatorcontrib>Atique, Rodrigo</creatorcontrib><creatorcontrib>Ishiy, Felipe Augusto André</creatorcontrib><creatorcontrib>Tissiani, Luiz Alexandre Lorico</creatorcontrib><creatorcontrib>Dalto Fanganiello, Roberto</creatorcontrib><creatorcontrib>Aguena, Meire</creatorcontrib><creatorcontrib>Passos-Bueno, Maria Rita</creatorcontrib><title>Optimization of Parameters for a More Efficient Use ofAdipose-Derived Stem Cells in Regenerative Medicine Therapies</title><title>Stem cells international</title><description>Adipose tissue-derived stem cells (ASCs) association to fat in autologous lipotransfer is promising for a more effective soft tissue reconstruction, and optimization of protocols to isolate ASCs from lipoaspirate fat is much needed. We demonstrated that an increase in adipocyte differentiation is dependent on the number of ASCs. In a sample of 10 donors, we found a higher concentration of nucleated cells in the lower abdomen compared to flank (P=0.015). In a sample of 6 donors we did not find differences in the cell yield obtained by manual or pump-assisted aspiration (P=0.56). We suggest that the increase in the number of ASCs in the reinjected fat may enhance the efficiency of newly formed adipose tissue and that the anatomical region from which to harvest fat tissue needs to be considered to optimize the number of ASCs in the harvested tissue. Finally, pump-assisted aspiration can be used without any significant harm to the viability of cells.</description><issn>1687-966X</issn><issn>1687-9678</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNqFTsFKAzEUDKJgsT15l_cDa5ON3W2PUiteimIreCvBneiT3WTJCwX9enMQr85lhmFmGKUujb42ZrGY19rUc6ttY_SJmphm2Varpl2e_unm9VzNRD51gV3pG11PlDyOmQf-dpljoOjpySU3ICMJ-ZjI0TYm0MZ7fmOETC-CErvteIyC6g6Jj-holzHQGn0vxIGe8Y6AVDaPoC26Ug2g_UexRoZM1Zl3vWD2yxfq6n6zXz9UGFyCd4cxcVFfB6NtudrafwM_HyxNvA</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>Alonso, Nivaldo</creator><creator>Atique, Rodrigo</creator><creator>Ishiy, Felipe Augusto André</creator><creator>Tissiani, Luiz Alexandre Lorico</creator><creator>Dalto Fanganiello, Roberto</creator><creator>Aguena, Meire</creator><creator>Passos-Bueno, Maria Rita</creator><general>Hindawi Publishing Corporation</general><scope>ADJCN</scope><scope>AHFXO</scope></search><sort><creationdate>2012</creationdate><title>Optimization of Parameters for a More Efficient Use ofAdipose-Derived Stem Cells in Regenerative Medicine Therapies</title><author>Alonso, Nivaldo ; Atique, Rodrigo ; Ishiy, Felipe Augusto André ; Tissiani, Luiz Alexandre Lorico ; Dalto Fanganiello, Roberto ; Aguena, Meire ; Passos-Bueno, Maria Rita</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-emarefa_primary_10300073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alonso, Nivaldo</creatorcontrib><creatorcontrib>Atique, Rodrigo</creatorcontrib><creatorcontrib>Ishiy, Felipe Augusto André</creatorcontrib><creatorcontrib>Tissiani, Luiz Alexandre Lorico</creatorcontrib><creatorcontrib>Dalto Fanganiello, Roberto</creatorcontrib><creatorcontrib>Aguena, Meire</creatorcontrib><creatorcontrib>Passos-Bueno, Maria Rita</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><jtitle>Stem cells international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alonso, Nivaldo</au><au>Atique, Rodrigo</au><au>Ishiy, Felipe Augusto André</au><au>Tissiani, Luiz Alexandre Lorico</au><au>Dalto Fanganiello, Roberto</au><au>Aguena, Meire</au><au>Passos-Bueno, Maria Rita</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optimization of Parameters for a More Efficient Use ofAdipose-Derived Stem Cells in Regenerative Medicine Therapies</atitle><jtitle>Stem cells international</jtitle><date>2012</date><risdate>2012</risdate><volume>2012</volume><issue>2012</issue><spage>1</spage><epage>7</epage><pages>1-7</pages><issn>1687-966X</issn><eissn>1687-9678</eissn><abstract>Adipose tissue-derived stem cells (ASCs) association to fat in autologous lipotransfer is promising for a more effective soft tissue reconstruction, and optimization of protocols to isolate ASCs from lipoaspirate fat is much needed. We demonstrated that an increase in adipocyte differentiation is dependent on the number of ASCs. In a sample of 10 donors, we found a higher concentration of nucleated cells in the lower abdomen compared to flank (P=0.015). In a sample of 6 donors we did not find differences in the cell yield obtained by manual or pump-assisted aspiration (P=0.56). We suggest that the increase in the number of ASCs in the reinjected fat may enhance the efficiency of newly formed adipose tissue and that the anatomical region from which to harvest fat tissue needs to be considered to optimize the number of ASCs in the harvested tissue. Finally, pump-assisted aspiration can be used without any significant harm to the viability of cells.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><doi>10.1155/2012/303610</doi><tpages>7</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1687-966X |
ispartof | Stem cells international, 2012, Vol.2012 (2012), p.1-7 |
issn | 1687-966X 1687-9678 |
language | eng |
recordid | cdi_emarefa_primary_1030007 |
source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Wiley Online Library Open Access; PubMed Central; Alma/SFX Local Collection |
title | Optimization of Parameters for a More Efficient Use ofAdipose-Derived Stem Cells in Regenerative Medicine Therapies |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T18%3A04%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-emarefa&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Optimization%20of%20Parameters%20for%20a%20More%20Efficient%20Use%20ofAdipose-Derived%20Stem%20Cells%20in%20Regenerative%20Medicine%20Therapies&rft.jtitle=Stem%20cells%20international&rft.au=Alonso,%20Nivaldo&rft.date=2012&rft.volume=2012&rft.issue=2012&rft.spage=1&rft.epage=7&rft.pages=1-7&rft.issn=1687-966X&rft.eissn=1687-9678&rft_id=info:doi/10.1155/2012/303610&rft_dat=%3Cemarefa%3E1030007%3C/emarefa%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |