Optimization of Parameters for a More Efficient Use ofAdipose-Derived Stem Cells in Regenerative Medicine Therapies

Adipose tissue-derived stem cells (ASCs) association to fat in autologous lipotransfer is promising for a more effective soft tissue reconstruction, and optimization of protocols to isolate ASCs from lipoaspirate fat is much needed. We demonstrated that an increase in adipocyte differentiation is de...

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Veröffentlicht in:Stem cells international 2012, Vol.2012 (2012), p.1-7
Hauptverfasser: Alonso, Nivaldo, Atique, Rodrigo, Ishiy, Felipe Augusto André, Tissiani, Luiz Alexandre Lorico, Dalto Fanganiello, Roberto, Aguena, Meire, Passos-Bueno, Maria Rita
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container_end_page 7
container_issue 2012
container_start_page 1
container_title Stem cells international
container_volume 2012
creator Alonso, Nivaldo
Atique, Rodrigo
Ishiy, Felipe Augusto André
Tissiani, Luiz Alexandre Lorico
Dalto Fanganiello, Roberto
Aguena, Meire
Passos-Bueno, Maria Rita
description Adipose tissue-derived stem cells (ASCs) association to fat in autologous lipotransfer is promising for a more effective soft tissue reconstruction, and optimization of protocols to isolate ASCs from lipoaspirate fat is much needed. We demonstrated that an increase in adipocyte differentiation is dependent on the number of ASCs. In a sample of 10 donors, we found a higher concentration of nucleated cells in the lower abdomen compared to flank (P=0.015). In a sample of 6 donors we did not find differences in the cell yield obtained by manual or pump-assisted aspiration (P=0.56). We suggest that the increase in the number of ASCs in the reinjected fat may enhance the efficiency of newly formed adipose tissue and that the anatomical region from which to harvest fat tissue needs to be considered to optimize the number of ASCs in the harvested tissue. Finally, pump-assisted aspiration can be used without any significant harm to the viability of cells.
doi_str_mv 10.1155/2012/303610
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title Optimization of Parameters for a More Efficient Use ofAdipose-Derived Stem Cells in Regenerative Medicine Therapies
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