1386PD - Longitudinal increase in Ki67 and high-grade transformation in pancreatic neuroendocrine tumours (PNETs)

Tumor proliferation and grade are important prognostic factors at diagnosis of PNETs. Little is known about how these factors change over time and if longitudinal increase in Ki67-index is associated with prognosis. The purpose of this work was to describe longitudinal changes in Ki67-index and tumor grade, as well as its impact on overall survival, in PNETs. Platelet free plasma from 68 GEP-NET patients was collected. Whole miRNOME NGS analysis was performed on exome enriched small-RNAs fraction of 24 patients: 12 18PET/FDG+ and 12 18PET/FDG-. MiRNAs significantly associated with 18PET/FDG outcome has been identified and validated by RT/qPCR on overall case series. Target miRNAs fold enrichment were then combined to create predictors (pAB, pAC, pBC and pABC). Man-Whitney test was applied and validated target miRNAs had been correlated with clinical outcome and clinical parameters (ki-67, grading, tumor burden and 68Gallium-PET SUVmax). NGS whole miRNOME analysis revealed 10 target miRNAs able to distinguish 18FDG/PET positive from negative Pancreatic-NETs (PNETs). Subsequently, mir-A, mir-B, mir-C (patent pending) have been validated on overall case series by multiplex RT/qPCR (p