Synthesis and preliminary evaluation of an18 F-labeled oleic acid analog for PET imaging of fatty acid uptake and metabolism

Abstract Introduction Imaging fatty acid uptake and utilization has broad impact in investigating myocardial diseases, hepatic functions, tumor progression, and the metabolic state of adipose tissue. The SPECT tracer123 I-15-( p -iodophenyl)-3-( R , S )-methylpentadecanoic acid (BMIPP) is a clinically used nuclear medicine tracer to image myocardial uptake of fatty acid. Although FTHA (18 F- 5 ) has been in clinical use for PET imaging of adipose tissue as well as the myocardium, here we developed a click oleate analog to compare to FTO, with the goal of improved stability to defluorination and suitability for imaging myocardial uptake and oxidation of fatty acids. Methods A rapid and convenient synthetic approach for a precursor to a18 F-labeled oleate analog using click chemistry was developed and evaluated for PET imaging in fasted mice. Results The overall yield for the preparation of the labeling precursor of the clicked oleate analog was 12%. This precursor was efficiently radiolabeled with F-18 in 17% non-decay-corrected radiochemical yield. PET/CT imaging and biodistribution results show that this fatty acid analog had reasonable heart uptake (0.94 ± 0.28 %ID/g at 0.5 h p.i.) and heart-to-muscle ratio (2.05 ± 0.39 at 0.5 h p.i.) and is a potential lead for developing new PET tracers to image fatty acid uptake and utilization using click chemistry methodologies. The synthetic route to FTO was optimized to three steps from known starting materials. Conclusion While the uptake of the clicked oleic acid analog was sufficient for visualizing the myocardium in mice, the preliminary metabolism data suggest that only a fraction of the uptake was due to fatty acid beta-oxidation. Studies are under way to explore the uptake/oxidation mechanism and kinetics.