Anticonvulsant activity of pregabalin in the maximal electroshock-induced seizure assay in α2 δ1 (R217A) and α2 δ2 (R279A) mouse mutants

Highlights • Mutant mice were made expressing α2 δ1 R217A and α2 δ2 R279A. • Phenytoin activity in maximal electroshock is not affected in these mutants. • Pregabalin reduces seizure severity and latency to seizures in wild type mice. • Activity of pregabalin is not blocked in the α2 δ2 mutant, but is in the α2 δ1 mutant. • The anticonvulsant action of pregabalin requires binding to α2 δ1 , not α2 δ2.