ICBP90 Regulation of DNA Methylation, Histone Ubiquitination, and Tumor Suppressor Gene Expression in Breast Cancer Cells

In mammals and other higher eukaryotes, SRA-RING proteins are essential for global maintenance of DNA methylation. This research project focuses on the discovery of new ubiquitination targets for the Arabidopsis SRA-RING protein VIM1 and the human SRA-RING protein UHRF1. Additionally, it will closely examine the methylcytosinebinding specificity of UHRF1, with a specific focus on non-CpG contexts. The proposed work is ongoing, and so far the major accomplishments include creation of relevant plant lines and development of in vitro assays. The original document contains color images.