Real-Time Fluorogenic Polymerase Chain Reaction (PCR) Assays to Nucleic Acid-Based Detection of Simulants and Biothreat Agents

Polymerase chain reaction (PCR) is an exquisitely sensitive method for the amplification and detection of genetic material (DNA and RNA sequences). We have developed several assays using a variation of PCR that proceeds very quickly and allows the monitoring of the progress of the reaction in real t...

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Bibliographische Detailangaben
Hauptverfasser:	Khan, Akbar S, O'Connell, Kevin P, Bucher, Jennifer R, Anderson, Patricia E, Cao, Cheng J, Gostomski, Mark V, Valdes, James J
Format:	Report
Sprache:	eng
Schlagworte:	BACTERIA BIOLOGICAL AGENTS CHAIN REACTIONS CHEMICAL AGENT SIMULANTS CODING DEOXYRIBONUCLEIC ACIDS DETECTION ENTEROTOXINS FOOD POISONING GENES Microbiology NUCLEIC ACIDS Polymer Chemistry POLYMERIZATION REAL TIME RIBONUCLEIC ACIDS STAPHYLOCOCCUS STAPHYLOCOCCUS AUREUS Toxicology TOXINS AND ANTITOXINS
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Beschreibung
Zusammenfassung:	Polymerase chain reaction (PCR) is an exquisitely sensitive method for the amplification and detection of genetic material (DNA and RNA sequences). We have developed several assays using a variation of PCR that proceeds very quickly and allows the monitoring of the progress of the reaction in real time. We describe here assays for the detection of the gene encoding staphylococcal enterotoxin A (SEA), a toxin produced by the bacterium Staphylococcus aureus and an agent responsible for a significant fraction of food poisoning incidents worldwide. We also describe several assays for the detection of a simulant of viral pathogens, the bacteriophage MS2. See also ADM001523.