Phospholipase A2(PLA2) Regulates Neuroexocytosis to Counteract Botulinum Toxin A (BONT/A) Poisoning

In this study, BoNT/A is one of the most serious biological threats faced by the U. S. military and allied forces. Previously we reported that in nerve growth factor (NGF)-differentiated PC12 cells, arachidonic acid (AA) release is associated with acetylcholine (ACh) release and BoNT/A inhibits both...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	Zhang, Xieping, Ishida, Hiroshi, Ray, Prabhati
Format:	Report
Sprache:	eng
Schlagworte:	ACETYLCHOLINE ARACHIDONIC ACID BACTERIAL TOXINS Biochemistry BONT/A(BOTULINUM TOXIN TYPE A) CLOSTRIDIUM BOTULINUM ENZYMES EXOCYTOSIS INHIBITION PHOSPHOLIPASE A2 PHOSPHOLIPIDS SYMPOSIA Toxicology
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	In this study, BoNT/A is one of the most serious biological threats faced by the U. S. military and allied forces. Previously we reported that in nerve growth factor (NGF)-differentiated PC12 cells, arachidonic acid (AA) release is associated with acetylcholine (ACh) release and BoNT/A inhibits both. We report the effect of PLA2 over expression on inhibition of ACh exocytosis due to BoNT/A light chain (LC) in PC12 cells. Over expression of PLA2 alone augmented the stimulated release of ACh and AA. PLA2 over expression also effectively prevented the inhibition of stimulated ACh and AA release due to BoNT/A LC. See also ADM001523. Presented at the Joint Service Scientific Conference on Chemical & Biological Defense Research held on 19-21 November 2002. The original document contains color images.