Microarray-based Resequencing of Multiple Bacillus anthracis Isolates

Microarray-based Resequencing of Multiple Bacillus anthracis Isolates

We used custom-designed resequencing arrays to generate 3.1 Mb of genomic sequence from a panel of 56 Bacillus anthracis strains. Sequence quality was shown to be very high by replication (discrepancy rate of 7.4 x 10(exp -7)) and by comparison to independently generated shotgun sequence (discrepanc...

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Bibliographische Detailangaben
Hauptverfasser: Zwick, Michael E, Mcafee, Farrell, Cutler, David J, Read, Timothy D, Ravel, Jacques, Bowman, Gregory R, Galloway, Darrell R, Mateczun, Alfred
Format: Report
Sprache:eng
Schlagworte:
BACILLUS ANTHRACIS
BIOTERRORISM
Chemical, Biological and Radiological Warfare
DEOXYRIBONUCLEIC ACIDS
GENETIC MAPPING
Medicine and Medical Research
MICROARRAYS
Microbiology
Numerical Mathematics
OLIGONUCLEOTIDES
PATHOGENIC MICROORGANISMS
SEQUENCES(MATHEMATICS)
SNP(SINGLE NUCLEOTIDE POLYMORPHISMS)
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Zusammenfassung:We used custom-designed resequencing arrays to generate 3.1 Mb of genomic sequence from a panel of 56 Bacillus anthracis strains. Sequence quality was shown to be very high by replication (discrepancy rate of 7.4 x 10(exp -7)) and by comparison to independently generated shotgun sequence (discrepancy rate 2.5 x 10(exp -6)). Population genomics studies of microbial pathogens using rapid resequencing technologies such as resequencing arrays are critical for recognizing newly emerging or genetically engineered strains. Pub. in Genome Biology, v6 n1, 17 Dec 2004.