Resolution of the Spatial Distribution of the Bacteriophage MS2 Protein and RNA Components

Resolution of the Spatial Distribution of the Bacteriophage MS2 Protein and RNA Components

Small-angle neutron scattering (SANS) and the contrast variation technique were used to determine the spatial relationship of the individual components of the MS2 virion (protein shell and genomic RNA). Our results show that (a) the MS2 RNA is tightly compacted within the virion, confined to a radiu...

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Bibliographische Detailangaben
Hauptverfasser: Kuzmanovic, Deborah A, Elashvili, Iiya, Wick, Charles H, O'Connell, Catherine, Krueger, Susan
Format: Report
Sprache:eng
Schlagworte:
BACTERIOPHAGES
Biochemistry
CONTRAST
DETECTION
GENOME
Microbiology
MS2
NEUTRON SCATTERING
Organic Chemistry
PROTEINS
PURIFICATION
RIBONUCLEIC ACIDS
SANS(SMALL ANGLE NEUTRON SCATTERING)
SPATIAL DISTRIBUTION
VIRION
VIRUS DETECTION
VIRUS STRUCTURES
VIRUSES
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Zusammenfassung:Small-angle neutron scattering (SANS) and the contrast variation technique were used to determine the spatial relationship of the individual components of the MS2 virion (protein shell and genomic RNA). Our results show that (a) the MS2 RNA is tightly compacted within the virion, confined to a radius of 83 +/- lA and (0) the MS2 coat protein shell extends to a radius of 136 + 2A and has a thickness of 2l +/- lA. Therefore, the protein core is composed primarily of solvent, and the RNA core is compact.