Automated Assays for Superoxide Dismutase, Catalase, Glutathione Peroxidase, and Glutathione Reductase Activity

Automated assays for catalase, glutathione peroxidase, glutathione reductase, and superoxide dismutase are presented. The assay for catalase is based on the peroxidatic activity of the enzyme. The glutathione peroxidase and reductase assays measure the consumption of nicotinamide adenine dinucleotid...

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Bibliographische Detailangaben
Hauptverfasser:	Wheeler, Conrad R, Salzman, Jhaine A, Elsayed, Nabil M, Omaye, Stanley T, Korte, Don W , Jr
Format:	Report
Sprache:	eng
Schlagworte:	ADENINE AMIDES ANALYZERS ASSAYING AUTOMATION Biochemistry BLOOD PROTEINS CAT CATALASE CELLS(BIOLOGY) CLINICAL MEDICINE COBRAS ENDOGENOUS ANTIOXIDANT ENZYME CHEMISTRY ENZYMES GLUTATHIONE MANUAL OPERATION NICOTINE NUCLEOTIDES OXIDATION OXIDOREDUCTASES OXYGEN OXYGEN RADICAL PEROXIDASES REPRINTS SAVINGS SOD SUPEROXIDES TIME TOXICITY
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Beschreibung
Zusammenfassung:	Automated assays for catalase, glutathione peroxidase, glutathione reductase, and superoxide dismutase are presented. The assay for catalase is based on the peroxidatic activity of the enzyme. The glutathione peroxidase and reductase assays measure the consumption of nicotinamide adenine dinucleotide following the reduction of t-butyl hydroperoxide and oxidized glutathione, respectively. The assay for superoxide dismutase is based on the reduction of cytochrome c. All assays utilize the Cobras FARA clinical automated analyzer and provide considerable time savings over the manual assays. Reprints. (jg) Availability: Pub. in Analytical Biochemistry v184 p193-199, 1990. No copies furnished by DTIC/NTIS.