Purification of Erythropoietin by Ion-Exchange Chromatography

Purification of Erythropoietin by Ion-Exchange Chromatography

A method is given for the purification of erythropoietin from the filtrate of acidified, boiled plasma prepared from phenylhydrazine anemic rabbits utilizing, DEAE-cellulose ion-exchange columns. The active erythropoietic factor thus prepared has been partially characterized and shown to be an acidi...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Rambach, W A, Cooper, J A, Alt, H L
Format: Report
Sprache:eng
Schlagworte:
Biochemistry
CHROMATOGRAPHY
ERYTHROPOIETINS
GLYCOPROTEINS
HORMONES
ION EXCHANGE
PURIFICATION
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Beschreibung
Zusammenfassung:A method is given for the purification of erythropoietin from the filtrate of acidified, boiled plasma prepared from phenylhydrazine anemic rabbits utilizing, DEAE-cellulose ion-exchange columns. The active erythropoietic factor thus prepared has been partially characterized and shown to be an acidic glycoprotein of low molecular weight.