Obtaining Bacterial Allergens by Alkaline Extraction and Research on Their Physicochemical Properties

A common method of obtaining allergens from bacteria of the group of particularly dangerous infections and of certain representatives of enteric family is suggested as a result of the work carried out. The method consists in extraction from microbial cells of alkali-soluble components, their precipitation with acid dialysis and lyophilization. The protein part of the allergens mainly contained the fraction with a high molecular weight. Thermal treatment of certain allergens (tularemic and typhoid) was accompanied by the rupture of bond in the protein molecule, and separation of fractions with a lesser molecular weight; some retained their specific activity. Edited machine trans. of Zhurnal Mikrobiologii, Epidemiologii i Immunobiologii (USSR) v47 n10 p72-77 1970, by Frank C. Vaughn.