The Growth of Venezuelan Equine Encephalomyelitis Virus in Human Diploid Cell Strain WI-38

The Growth of Venezuelan Equine Encephalomyelitis Virus in Human Diploid Cell Strain WI-38

We demonstrated that Venezuelan equine encephalomyelitis (VEE) replicated in and adapted rapidly to human diploid cell strain WI-38. Peak titers of as high as 10 to the 9.9th power MICLD50/ml were obtained after a 48- hour incubation period at low passage levels in Eagle basal medium supplemented wi...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Reitman, Morton, Green, Leonard
Format: Report
Sprache:eng
Schlagworte:
AGGLUTININS
CELLS(BIOLOGY)
CULTURE MEDIA
GROWTH(PHYSIOLOGY)
Microbiology
PATHOLOGY
TISSUE CULTURE CELLS
VACCINES
VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:We demonstrated that Venezuelan equine encephalomyelitis (VEE) replicated in and adapted rapidly to human diploid cell strain WI-38. Peak titers of as high as 10 to the 9.9th power MICLD50/ml were obtained after a 48- hour incubation period at low passage levels in Eagle basal medium supplemented with 3% calf serum. VEE virus replicated poorly in serum-free medium. Propagation of the virus was accompanied by the production of hemagglutinin and cytopathogenic effect. These studies suggest that the WI-38 strain might be an appropriate source for the production of VEE virus for vaccines.