The Rapid Diagnosis of Acute Respiratory Disease Due to Adenovirus: Preparation and Standardization of Fluorescent Antibody Reagents
Fluorescent antibody reagents were prepared for the rapid diagnosis of acute respiratory disease caused by adenovirus in a military population. Methods are described for the preparation of high-titered antisera in rabbits, fractionation of globulins from the serum, conjugation with fluorescein isoth...
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| creator | McCormick, David P Galapon, Quirino Berling, Carl Smith, Edward P Combs, Raymond S |
| description | Fluorescent antibody reagents were prepared for the rapid diagnosis of acute respiratory disease caused by adenovirus in a military population. Methods are described for the preparation of high-titered antisera in rabbits, fractionation of globulins from the serum, conjugation with fluorescein isothiocyanate, and sorption to remove nonspecific staining. Fluorescein-tagged antisera were specific at diluations of 1:20 to 1:30. |
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Methods are described for the preparation of high-titered antisera in rabbits, fractionation of globulins from the serum, conjugation with fluorescein isothiocyanate, and sorption to remove nonspecific staining. Fluorescein-tagged antisera were specific at diluations of 1:20 to 1:30.</description><language>eng</language><subject>ADENOVIRUSES ; ANTIBODIES ; ANTIGENS ; FLUORESCENT ANTIBODY TECHNIQUES ; Medicine and Medical Research ; Microbiology ; RESPIRATORY DISEASES ; SERODIAGNOSIS ; TEST METHODS ; VIRUS DISEASES</subject><creationdate>1972</creationdate><rights>APPROVED FOR PUBLIC RELEASE</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,780,885,27567,27568</link.rule.ids><linktorsrc>$$Uhttps://apps.dtic.mil/sti/citations/AD0743082$$EView_record_in_DTIC$$FView_record_in_$$GDTIC$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>McCormick, David P</creatorcontrib><creatorcontrib>Galapon, Quirino</creatorcontrib><creatorcontrib>Berling, Carl</creatorcontrib><creatorcontrib>Smith, Edward P</creatorcontrib><creatorcontrib>Combs, Raymond S</creatorcontrib><creatorcontrib>NAVAL MEDICAL FIELD RESEARCH LAB CAMP LEJEUNE NC</creatorcontrib><title>The Rapid Diagnosis of Acute Respiratory Disease Due to Adenovirus: Preparation and Standardization of Fluorescent Antibody Reagents</title><description>Fluorescent antibody reagents were prepared for the rapid diagnosis of acute respiratory disease caused by adenovirus in a military population. Methods are described for the preparation of high-titered antisera in rabbits, fractionation of globulins from the serum, conjugation with fluorescein isothiocyanate, and sorption to remove nonspecific staining. Fluorescein-tagged antisera were specific at diluations of 1:20 to 1:30.</description><subject>ADENOVIRUSES</subject><subject>ANTIBODIES</subject><subject>ANTIGENS</subject><subject>FLUORESCENT ANTIBODY TECHNIQUES</subject><subject>Medicine and Medical Research</subject><subject>Microbiology</subject><subject>RESPIRATORY DISEASES</subject><subject>SERODIAGNOSIS</subject><subject>TEST METHODS</subject><subject>VIRUS DISEASES</subject><fulltext>true</fulltext><rsrctype>report</rsrctype><creationdate>1972</creationdate><recordtype>report</recordtype><sourceid>1RU</sourceid><recordid>eNqFjLEKwkAQRNNYiPoHFvsDQjCCYncYg6WofVhzm7gQ7sLtnhBrP9wD7W1mYN7wptn79iC44MAWSsbOeWEB34JpoiZAMnBA9WFMWAiFoIwE6sFYcv7JIcoezoEGTDf2DtBZuGpKDJZf3y35qj76QNKQUzBO-e7tmPTYpUHm2aTFXmjx61m2rI63w2lllZtalB1pbcp8uyny3br4gz8t70dk</recordid><startdate>197205</startdate><enddate>197205</enddate><creator>McCormick, David P</creator><creator>Galapon, Quirino</creator><creator>Berling, Carl</creator><creator>Smith, Edward P</creator><creator>Combs, Raymond S</creator><scope>1RU</scope><scope>BHM</scope></search><sort><creationdate>197205</creationdate><title>The Rapid Diagnosis of Acute Respiratory Disease Due to Adenovirus: Preparation and Standardization of Fluorescent Antibody Reagents</title><author>McCormick, David P ; Galapon, Quirino ; Berling, Carl ; Smith, Edward P ; Combs, Raymond S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-dtic_stinet_AD07430823</frbrgroupid><rsrctype>reports</rsrctype><prefilter>reports</prefilter><language>eng</language><creationdate>1972</creationdate><topic>ADENOVIRUSES</topic><topic>ANTIBODIES</topic><topic>ANTIGENS</topic><topic>FLUORESCENT ANTIBODY TECHNIQUES</topic><topic>Medicine and Medical Research</topic><topic>Microbiology</topic><topic>RESPIRATORY DISEASES</topic><topic>SERODIAGNOSIS</topic><topic>TEST METHODS</topic><topic>VIRUS DISEASES</topic><toplevel>online_resources</toplevel><creatorcontrib>McCormick, David P</creatorcontrib><creatorcontrib>Galapon, Quirino</creatorcontrib><creatorcontrib>Berling, Carl</creatorcontrib><creatorcontrib>Smith, Edward P</creatorcontrib><creatorcontrib>Combs, Raymond S</creatorcontrib><creatorcontrib>NAVAL MEDICAL FIELD RESEARCH LAB CAMP LEJEUNE NC</creatorcontrib><collection>DTIC Technical Reports</collection><collection>DTIC STINET</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>McCormick, David P</au><au>Galapon, Quirino</au><au>Berling, Carl</au><au>Smith, Edward P</au><au>Combs, Raymond S</au><aucorp>NAVAL MEDICAL FIELD RESEARCH LAB CAMP LEJEUNE NC</aucorp><format>book</format><genre>unknown</genre><ristype>RPRT</ristype><btitle>The Rapid Diagnosis of Acute Respiratory Disease Due to Adenovirus: Preparation and Standardization of Fluorescent Antibody Reagents</btitle><date>1972-05</date><risdate>1972</risdate><abstract>Fluorescent antibody reagents were prepared for the rapid diagnosis of acute respiratory disease caused by adenovirus in a military population. Methods are described for the preparation of high-titered antisera in rabbits, fractionation of globulins from the serum, conjugation with fluorescein isothiocyanate, and sorption to remove nonspecific staining. Fluorescein-tagged antisera were specific at diluations of 1:20 to 1:30.</abstract><oa>free_for_read</oa></addata></record> |
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| source | DTIC Technical Reports |
| subjects | ADENOVIRUSES ANTIBODIES ANTIGENS FLUORESCENT ANTIBODY TECHNIQUES Medicine and Medical Research Microbiology RESPIRATORY DISEASES SERODIAGNOSIS TEST METHODS VIRUS DISEASES |
| title | The Rapid Diagnosis of Acute Respiratory Disease Due to Adenovirus: Preparation and Standardization of Fluorescent Antibody Reagents |
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