The Rapid Diagnosis of Acute Respiratory Disease Due to Adenovirus: Preparation and Standardization of Fluorescent Antibody Reagents

The Rapid Diagnosis of Acute Respiratory Disease Due to Adenovirus: Preparation and Standardization of Fluorescent Antibody Reagents

Fluorescent antibody reagents were prepared for the rapid diagnosis of acute respiratory disease caused by adenovirus in a military population. Methods are described for the preparation of high-titered antisera in rabbits, fractionation of globulins from the serum, conjugation with fluorescein isoth...

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Bibliographische Detailangaben
Hauptverfasser: McCormick, David P, Galapon, Quirino, Berling, Carl, Smith, Edward P, Combs, Raymond S
Format: Report
Sprache:eng
Schlagworte:
ADENOVIRUSES
ANTIBODIES
ANTIGENS
FLUORESCENT ANTIBODY TECHNIQUES
Medicine and Medical Research
Microbiology
RESPIRATORY DISEASES
SERODIAGNOSIS
TEST METHODS
VIRUS DISEASES
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Beschreibung
Zusammenfassung:Fluorescent antibody reagents were prepared for the rapid diagnosis of acute respiratory disease caused by adenovirus in a military population. Methods are described for the preparation of high-titered antisera in rabbits, fractionation of globulins from the serum, conjugation with fluorescein isothiocyanate, and sorption to remove nonspecific staining. Fluorescein-tagged antisera were specific at diluations of 1:20 to 1:30.