The Detection of Sickle Cell Disease in Large Human Populations by an Automated Technique

A total of 2,939 specimens was tested in this mixed population of both military and civilian individuals. Complete agreement among the results of all tests was obtained. An automated test for the determination of S hemoglobin can be performed at the rate of 60 per hour per AutoAnalyzer unit on 0.5 ml of whole blood with accuracy and reliability on large human populations. The successful field trials we have conducted may be regarded as a prototype model for the survey of metropolitan and military populations leading to case-finding of sickle cell disease. These cases may then be directed to medical genetic counseling and for possible treatment with urea. This is the first large-scale human population survey by specific automated techniques diagnostic for the molecular lesion of S hemoglobin. The automated mass survey technique is proven and awaits immediate application to appropriate segments of our national population. This diagnostic technique appropriately fielded can yield substantial contributions to the development of accurate statistics and to the control and eventual elimination of this lethal genetic affliction, sickle cell disease. The impediment to the widespread use of this proven technique is the high cost per test due to the use of the proprietary Sickledex solution. (Author)