CULTIVATION OF VIRUS OF SPRING-SUMMER AND JAPANESE ENCEPHALIDES ON DEVELOPING CHICKEN EMBRYO

The possibility of successfully cultivating the virus of spring- summer and Japanese encephalitis in developing chicken embryo was established. The optimal conditions during cultivation of the virus of Japanese encephalitis are: 8-9 day embryos, infected in the yolk sac and incubated at 35.5 C for 48 hours. During such conditions of cultivation the virus is distributed in the eggs as follows: most in the brain of the embryo, then in the body of the embryo, in the allantois fluid, in the chorionallantois membrane and the least in the yolk membrane. The optimal conditions for the cultivation of the virus of spring-summer encephalitis are: infection of enbryos of 9-10 days incubation by applying the virus on the chorionallantois membrane and incubating at 37C for 72 hours. During this the greatest quantity of virus is detected in the brain and body of the embryo, in the yolk and chorionallantois membrane, the least- in the fluid and blood of the embryo. Trans. of Zhurnal Mikrobiologii, Epidemiologii i Immunobiologii (USSR) v26 n7 p64-69 1955.