DIFFERENTIATION BETWEEN PROTEOLYTIC AND TAME ESTEROLYTIC ACTIVITY IN CROTALUS ATROX VENOM

DIFFERENTIATION BETWEEN PROTEOLYTIC AND TAME ESTEROLYTIC ACTIVITY IN CROTALUS ATROX VENOM

Although Crotalus atrox venom can hydrolyze proteins and arginine esters, these activities are not due to a trypsin-like enzyme. Proteolytic and esterolytic activities have been differentiated by specific inhibition by such compounds as PMSF, DFP, EDTA and 2-mercaptoethanol, and by actual resolution...

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Bibliographische Detailangaben
Hauptverfasser: Bobbitt,Jesse D, Reed,Donald E
Format: Report
Sprache:eng
Schlagworte:
Biochemistry
CHROMATOGRAPHIC ANALYSIS
ENZYME INHIBITORS
ENZYMES
PEPTIDE HYDROLASES
PLASMIN
TRYPSIN
VENOMS
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Zusammenfassung:Although Crotalus atrox venom can hydrolyze proteins and arginine esters, these activities are not due to a trypsin-like enzyme. Proteolytic and esterolytic activities have been differentiated by specific inhibition by such compounds as PMSF, DFP, EDTA and 2-mercaptoethanol, and by actual resolution of the venom by DEAE-cellulose chromatography. Preliminary results suggest that the proteolytic activity in the venom may have a limited specificity and that this specificity is quite unlike that of trypsin and plasmin. (Author)