From a genome-wide screen of RNAi molecules against SARS-CoV-2 to a validated broad-spectrum and potent prophylaxis

From a genome-wide screen of RNAi molecules against SARS-CoV-2 to a validated broad-spectrum and potent prophylaxis

Expanding the arsenal of prophylactic approaches against SARS-CoV-2 is of utmost importance, specifically those strategies that are resistant to antigenic drift in Spike. Here, we conducted a screen of over 16,000 RNAi triggers against the SARS-CoV-2 genome, using a massively parallel assay to ident...

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Veröffentlicht in:Communications biology 2023-03, Vol.6 (1), p.277-277, Article 277
Hauptverfasser: Yogev, Ohad, Weissbrod, Omer, Battistoni, Giorgia, Bressan, Dario, Naamati, Adi, Falciatori, Ilaria, Berkyurek, Ahmet Can, Rasnic, Roni, Izuagbe, Rhys, Hosmillo, Myra, Ilan, Shaul, Grossman, Iris, McCormick, Lauren, Honeycutt, Christopher Cole, Johnston, Timothy, Gagne, Matthew, Douek, Daniel C, Goodfellow, Ian, Hannon, Gregory James, Erlich, Yaniv
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Sprache:eng
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Administration, Intranasal
Animals
Antigenic drift
Biology
Biomedical and Life Sciences
COVID-19 - prevention & control
Cricetinae
Disease prevention
Genomes
Infectivity
Intranasal administration
Life Sciences
Mesocricetus
Monoclonal antibodies
Prophylaxis
RNA Interference
RNA, Small Interfering - genetics
RNA, Small Interfering - therapeutic use
RNA-mediated interference
SARS-CoV-2 - genetics
Saturation mutagenesis
Severe acute respiratory syndrome coronavirus 2
siRNA
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Zusammenfassung:Expanding the arsenal of prophylactic approaches against SARS-CoV-2 is of utmost importance, specifically those strategies that are resistant to antigenic drift in Spike. Here, we conducted a screen of over 16,000 RNAi triggers against the SARS-CoV-2 genome, using a massively parallel assay to identify hyper-potent siRNAs. We selected Ten candidates for in vitro validation and found five siRNAs that exhibited hyper-potent activity (IC50 
ISSN:2399-3642
2399-3642
DOI:10.1038/s42003-023-04589-5