Antinociceptive and anti-inflammatory activities of ethanol-soluble acidic component from Ganoderma atrum by suppressing mannose receptor

[Display omitted] •The antinociceptive and anti-inflammatory activities of ESAC was observed.•ESAC-mediated antinociceptive effects were related to anti-inflammatory activities.•Opiate receptors were linked to the analgesic effect of ESAC in mice.•MR acted as vital roles for ESAC to execute anti-inflammatory response in macrophages. The current study aimed to investigate antinociceptive and anti-inflammatory activities of ethanol-soluble acidic component from Ganoderma atrum (ESAC) and possible mechanisms involved. Our results showed that ESAC had significant abilitiestorelieve pain caused by thermal stimulation in mice. In the writhing response, ESAC could reduce the number of writhing responses induced by acetic acid in mice. Moreover, naloxone antagonized analgesic effects of ESAC and morphine, indicating that their analgesic effects might be achieved through opioid receptors. Subsequently, ESAC were found to control aberrant inflammation by reducing phagocytosis, nitric oxide (NO) and IL-1β in Lipopolysaccharides (LPS)-stimulated macrophages. Meanwhile, ESAC up-regulated anti-inflammatory parameters IL-10 and mannose receptor (MR). Furthermore, a combination of MR inhibitor and ESAC significantly blocked these anti-inflammatory activities compare with ESAC alone. Collectively, ESAC was demonstrated to perform antinociceptive and anti-inflammatory capacities, indicating that MR played a key role in ESAC-mediated anti-inflammatory activities.