Long non-coding RNA-PVT1 induces the progression of pulmonary fibrosis via the JAK/STAT3 signaling pathway

Objective To investigate the role of long non-coding RNA-PVT1/JAK/STAT3 signaling pathway in pulmonary fibrosis. Methods BEAS-2B cells were treated with different concentrations of LPS. Cell viability was assessed by CCK-8. The relative expression level of α-SMA protein was determined by western blot. The concentration of LPS was screened. BEAS-2B cells were treated with the optimal concentration of LPS to establish the pulmonary fibrosis model in vitro. BEAS-2B cells were transiently transfected with lnc-PVT1 siRNA and control siRNA followed by LPS treatment. The expression level of lnc-PVT1 was determined by RT-qPCR. Effective inhibitory targets were selected for subsequent experiment. BEAS-2B cells were divided into the control （Con）group, LPS group, LPS+siRNA control （LPS+siNC） group and LPS+siPVT1 group. After transiently transfected with siPVT1 and siRNA control, BEAS-2B cells were treated with 10μg/mL LPS for 24 h. Cell viability was assessed by CCK-8. Cell migration was evaluated by cell scratch test.