Expression of adhA from different organisms in Clostridium thermocellum
is a cellulolytic anaerobic thermophile that is a promising candidate for consolidated bioprocessing of lignocellulosic biomass into biofuels such as ethanol. It was previously shown that expressing in increases ethanol yield.In this study, we investigated expression of genes from different organism...
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| Veröffentlicht in: | Biotechnology for biofuels 2017-11, Vol.10 (1), p.251-251, Article 251 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
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| Zusammenfassung: | is a cellulolytic anaerobic thermophile that is a promising candidate for consolidated bioprocessing of lignocellulosic biomass into biofuels such as ethanol. It was previously shown that expressing
in
increases ethanol yield.In this study, we investigated expression of
genes from different organisms in
.
Based on sequence identity to
, we chose
genes from 10 other organisms:
,
,
,
,
strain AN1,
,
,
,
, and
. All 11
genes (including
) were expressed in
and fermentation end products were analyzed.
All 11
genes increased
ethanol yield compared to the empty-vector control.
and
genes generated significantly higher ethanol yield than
.
Our results indicated that expressing
is an effective method of increasing ethanol yield in wild-type
, and that this appears to be a general property of
genes. |
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| ISSN: | 1754-6834 1754-6834 |
| DOI: | 10.1186/s13068-017-0940-8 |