Genotoxic Effects of Rice-Agrochemicals on Channa punctatus (Bloch) and Cyprinus carpio (Linnaeus) Using Micronucleus Assay and Alkaline Single Cell Gel Electrophoresis

Rice-cum-fish culture is a cost-effective practice for marginal farmers but the major constraint is the indiscriminate use of agrochemicals. Present work was designed to assess the genotoxic effects of rice agrochemicals in Channa punctatus (Experiment 1 CP1 to CP3) and Cyprinus carpio (Experiment 2...

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Veröffentlicht in:	Nature environment and pollution technology 2022-12, Vol.21 (4), p.1807-1815
Hauptverfasser:	Kaur, M., Bhatnagar, A., Dhillon, O., Yadav, A. S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aberration Agrochemicals agrochemicals, dna damage, genotoxicity, micronucleus assay, rice-cum-fish culture Aquaculture Bioassays Biomarkers Cell culture Cell division Channa punctata Chromosome aberrations Chromosomes Comet assay Deoxyribonucleic acid Digital cameras DNA DNA damage Electrophoresis Experiments Farmers Fish Fish culture Food Gel electrophoresis Genotoxicity Micronuclei Pesticides Rice Toxicity Variance analysis
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Zusammenfassung:	Rice-cum-fish culture is a cost-effective practice for marginal farmers but the major constraint is the indiscriminate use of agrochemicals. Present work was designed to assess the genotoxic effects of rice agrochemicals in Channa punctatus (Experiment 1 CP1 to CP3) and Cyprinus carpio (Experiment 2 CC1 to CC3); using micronucleus, chromosome aberration, and single cell gel electrophoresis/Comet assay. Two experiments with three treatments (CP1/CC1: without pesticide; CP2/CC2: recommended doses; CP3/CC3: farmers’ dose) were maintained in triplicates. The presence of tail DNA and micronuclei depicted significant DNA damage (P
ISSN:	2395-3454 0972-6268 2395-3454
DOI:	10.46488/NEPT.2022.v21i04.035