Icariin promotes osteogenic differentiation by upregulating alpha-enolase expression

Osteogenic differentiation is a crucial biological process for maintaining bone remodelling. Aerobic glycolysis is the main source of energy for osteogenic differentiation. Alpha-enolase (Eno1), a glycolytic enzyme, is a therapeutic target for numerous diseases. Icariin, a principal active component of the traditional Chinese medicine Epimedium grandiflorum, can stimulate osteogenic differentiation. Here, we aimed to determine if icariin promotes osteogenic differentiation via Eno1. Icariin (1 μM) significantly promoted osteogenic differentiation of MC3T3-E1 cells. Icariin upregulated Eno1 protein and gene expressions during osteogenic differentiation. Moreover, ENOblock, a specific inhibitor of Eno1, markedly inhibited icariin-induced osteogenic differentiation. Futhermore, western blot assay showed that Eno1 might mediate osteogenic differentiation through the BMP/Smad4 signalling pathway. Collectively, Eno1 could be a promising drug target for icariin to regulate osteogenic differentiation. •Icariin remarkedly promotes the osteoblast differentiation of MC3T3-E1 cells.•Alpha-enolase (Eno1) is a potential target to regulate the osteoblast differentiation.•Icariin promotes the osteoblast differentiation via the upregulation of Eno1.•BMP/Smad4 signalling pathway plays a vital role on Eno1-mediated osteoblast differentiation.