Sulfate-Reducing Bacteria Isolated from an Oil Field in Kazakhstan and a Description of Pseudodesulfovibrio karagichevae sp. nov

Sulfidogenic bacteria cause numerous issues in the oil industry since they produce sulfide, corroding steel equipment, reducing oil quality, and worsening the environmental conditions in oil fields. The purpose of this work was to isolate and taxonomically identify the sulfidogenic bacteria responsible for the corrosion of steel equipment at the Karazhanbas oil field (Kazakhstan). In this study, we characterized five sulfidogenic strains of the genera , , and isolated from the formation water of the Karazhanbas oil field (Kazakhstan). Sulfate-reducing strain 9FUS revealed 98.9% similarity of the 16S rRNA gene sequence with the closely related strain ' ' 5S69 and was studied in detail to enhance the taxonomic resolution. Strain 9FUS grew optimally at 23-28 °C, pH 6.5, and 0-2% ( / ) NaCl. The strain used lactate, pyruvate, methanol, ethanol, fructose, ribose, and H /CO (in the presence of acetate) as carbon and energy sources for sulfate reduction. -C ω11, C , -C , and C were the predominant fatty acids. The genome is 4.20 Mbp with a G + C content of 64.0%. The average nucleotide identity and digital DNA-DNA hybridization values with spp. genomes were 72.5-91.6% (