Molecular Detection of Multidrug Resistant Salmonella Species Isolated from Broiler Farm in Bangladesh

Multidrug resistant (MDR) are a leading cause of foodborne diseases and serious human health concerns worldwide. In this study we detected MDR in broiler chicken along with the resistance genes and class 1 integron gene . A total of 100 samples were collected from broiler farms comprising 50 cloacal swabs, 35 litter and 15 feed samples. Overall prevalence of was 35% with the highest detected in cloacal swabs. Among the , 30 isolates were confirmed as serovar Typhimurium using molecular methods of PCR. Disk diffusion susceptibility test revealed that all the were classified as MDR with the highest resistance to tetracycline (97.14%), chloramphenicol (94.28%), ampicillin (82.85%) and streptomycin (77.14%). The most prevalent resistance genotypes were (97.14%), (94.28%), (82.85%) and (77.14%). In addition, among the MDR 20% were positive for class 1 integron gene ( . As far as we know, this is the first study describing the molecular basis of antibiotic resistance in MDR from broiler farms in Bangladesh. In addition to , and were also detected in the isolated MDR The detection of MDR in broiler chicken carrying is of serious public health concern because of their zoonotic nature and possibilities to enter into the food chain.