Quantitative Phase Contrast Microscopy with Optimized Partially Coherent Illumination

This paper presents a partially coherent illumination quantitative phase contrast microscopic (PCI-QPCM) prototype. In the PCI-QPCM prototype, the light scattered by a rotating diffuser is coupled into a multi-mode fiber, and the output light is used as the illumination for PCI-QPCM. The illuminatio...

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Veröffentlicht in:Photonics 2023-04, Vol.10 (4), p.391
Hauptverfasser: Zhuo, Kequn, Wang, Yang, Ma, Ying, An, Sha, Zalevsky, Zeev, Zheng, Juanjuan, Gao, Peng
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Sprache:eng
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Zusammenfassung:This paper presents a partially coherent illumination quantitative phase contrast microscopic (PCI-QPCM) prototype. In the PCI-QPCM prototype, the light scattered by a rotating diffuser is coupled into a multi-mode fiber, and the output light is used as the illumination for PCI-QPCM. The illumination wave has a constrained spectrum with a diameter of tens of micrometers, which can reduce speckle noise and will not broaden the dc term of the object wave. In the Fourier plane of the object wave, grating-masked phase shifters generated by a spatial light modulator (SLM) allow for measuring the intensity of the undiffracted and diffracted components of the object wave, as well as the phase-shifted interference patterns of the two. Quantitative phase images can be reconstructed from the recorded intensity images. The proposed PCI-QPCM was demonstrated with quantitative phase imaging of a transparent waveguide and a phase-step sample.
ISSN:2304-6732
2304-6732
DOI:10.3390/photonics10040391