Running speed and REM sleep control two distinct modes of rapid interhemispheric communication

Rhythmic gamma-band communication within and across cortical hemispheres is critical for optimal perception, navigation, and memory. Here, using multisite recordings in both rats and mice, we show that even faster ~140 Hz rhythms are robustly anti-phase across cortical hemispheres, visually resembling splines, the interlocking teeth on mechanical gears. Splines are strongest in superficial granular retrosplenial cortex, a region important for spatial navigation and memory. Spline-frequency interhemispheric communication becomes more coherent and more precisely anti-phase at faster running speeds. Anti-phase splines also demarcate high-activity frames during REM sleep. While splines and associated neuronal spiking are anti-phase across retrosplenial hemispheres during navigation and REM sleep, gamma-rhythmic interhemispheric communication is precisely in-phase. Gamma and splines occur at distinct points of a theta cycle and thus highlight the ability of interhemispheric cortical communication to rapidly switch between in-phase (gamma) and anti-phase (spline) modes within individual theta cycles during both navigation and REM sleep. Gamma-rhythmic communication within and across cortical hemispheres is critical for optimal perception, navigation, and memory. Here, Ghosh et al. identify even faster ~140 Hz rhythms, named splines, that reflect anti-phase neuronal synchrony across hemispheres. The balance of anti-phase spline and in-phase gamma communication is dynamically controlled by behavior and sleep.