Isolation and Characterization of Antibiotic Resistant Bacteria from Swiftlet Feces in Swiftlet Farm Houses in Sarawak, Malaysia

There is a growing concern on the occurrence of antimicrobial resistance. Development of multiple antibiotic resistant bacteria has overtaken new drug development and threatened the patients with untreatable infections. This study was conducted to isolate and characterize the antibiotic resistant bacteria from swiftlet farm houses located in various places including Kota Samarahan, Semarang, Saratok, Betong, Sarikei, Sibu, Sepinang, Maludam, Miri, and Kuching in Sarawak, Malaysia. Five feces samples were collected randomly from each site. One gram of the feces sample was diluted in 9 mL of 0.85% normal saline solution. The diluted sample was plated on Trypticase Soy agar plates and incubated at 37±1 °C for 24 h. A total of 500 bacteria isolates were then identified using 16S rRNA analysis method. Disc diffusion method was then used to confirm the resistant phenotypes of these isolates. The results showed that the means of the bacterial colony count were significantly different (p