Quantification of Salicylic Acid (SA) and SA-glucosides in Arabidopsis thaliana

Quantification of Salicylic Acid (SA) and SA-glucosides in Arabidopsis thaliana

Homeostasis between the cytoplasmic plant hormone salicylic acid (SA) and its' inactive, vacuolar storage forms, SA-2- β-D-glucoside (SAG) and SA-β-D-Glucose Ester (SGE), regulates the fine-tuning of defense responses to biotrophic pathogens in . This protocol describes a simplified, optimized...

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Veröffentlicht in:Bio-protocol 2018-05, Vol.8 (10), p.e2844-e2844
Hauptverfasser: Allasia, Valérie, Industri, Benoit, Ponchet, Michel, Quentin, Michaël, Favery, Bruno, Keller, Harald
Format: Artikel
Sprache:eng
Schlagworte:
Biology
Life Sciences
Methods
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Zusammenfassung:Homeostasis between the cytoplasmic plant hormone salicylic acid (SA) and its' inactive, vacuolar storage forms, SA-2- β-D-glucoside (SAG) and SA-β-D-Glucose Ester (SGE), regulates the fine-tuning of defense responses to biotrophic pathogens in . This protocol describes a simplified, optimized procedure to extract and quantify free SA and total hydrolyzable SA in plant tissues using a classical HPLC-based method.
ISSN:2331-8325
2331-8325
DOI:10.21769/bioprotoc.2844