Phenotypic evaluation of DH-lines developed on the base of rice samples with blast resistance genes

Gametoclonal changes are an additional source of genetic variation suitable for use in crop improvement programs for a range of agronomic traits. The aim of the study was to accelerate development of genetically stable DH rice lines with high morphological and genetic uniformity by the method of experimental haploidy in vitro based on breeding valuable samples with specified characteristics and carrying genes of a wide spectrum of blast resistance, as well as samples with racespecific genes for resistance to the pathogen, and subsequent assessment of agronomic traits of androgenic lines. The data on the callusogenic and regenerative capacity of 30 genotypes of domestic rice and samples of Chinese introduction with genes for blast resistance in anther culture in vitro are presented. Genetically stable (homozygous) androgenic lines were developed on the basis of these breeding valuable samples. The characteristics of economic and biological traits and elements of panicle productivity of 30 DH-line developed from three samples with blast resistance genes are given. The variability of traits arising as a result of gametoclonal variability in androgenic lines was noted for such traits as plant height, panicle length, blast resistance, and weight of 1000 grains.